http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-S6447800-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_129e393582e6bdf4029baf2206522f45 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00 |
| filingDate | 1987-08-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bfdef14bb39106917a9fb901f06ab632 |
| publicationDate | 1989-02-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-S6447800-A |
| titleOfInvention | Purification of human lipocortin i |
| abstract | PURPOSE:To purity the titled compound useful as an anti-inflammatory agent, etc., on an industrial scale at a low cost, by contacting a solution of human lipocortin I with a calcium-based adsorbent, eluting the adsorbed material, contacting with a anion exchange material, eluting the adsorbed material, contacting with a carrier bonded with a red pigment and finally eluting the adsorbed component. CONSTITUTION:An E.coli cell is transformed by inserting a manifestation plasmid integrated with a human lipocortin I structure gene separated from a fibroblast cDNA library and the obtained recombinant E.coli is cultured to obtain crude human lipocortin I solution. The solution is brought into contact with a calcium-based adsorbent, eluted, contacted with an anion exchange material and eluted. The eluted component is brought into contact with a carrier bonded with a red pigment or a blue pigment and eluted to obtain highly purified human lipocortin I. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-2308380-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-2308380-B |
| priorityDate | 1987-08-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 36.