http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-S6440000-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9032cdad64660442be1ad614c9e59495 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 |
| filingDate | 1987-08-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_71a74eef7df81df2edcb7b30c87d6571 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_74c6c993b8c0923ff8387c648639fcc5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0e91ecbf845fa2755d1d1b180940133c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0432a1c5fdbe48eb3bd7c7e166578da4 |
| publicationDate | 1989-02-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-S6440000-A |
| titleOfInvention | Method for fractionating and detecting hop stunt viroids |
| abstract | PURPOSE:To readily obtain hop stunt viroid such as hop stunt viroid cucumber variant useful in diagnosis of a plant pathogen, by detecting and fractionating the viroid with a specific DNA probe. CONSTITUTION:DNA is synthesized according to phosphotriester method to 15-25 mer synthetic DNA probe (A) containing a base sequence expressed by the formula (a is adenine, G is guanine, C is cytosine and T is thymine) and having complementarily with RNA of hop stunt viroid grape variant (HSV-g). Then a cucumber each infected to hop stunt viroid (HSV), hop stunt cucumber variant (HSV-c) and HSV-g is extracted to afford low-molecular RNA fraction (B) containing each viroid. Then components A and B are subjected to hybridimerization while changing temperature condition and strength and weakness of the reaction is labeled with radioisotope (e.g. <32>P) label to fractionate and provide the hop stunt viroids of HSV, HSV-g and HSV-g. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8551439-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7619080-B2 |
| priorityDate | 1987-08-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 35.