http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-S6391091-A

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filingDate 1986-10-07-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a86e950f131df4ab170dc6b3c22719b6
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publicationDate 1988-04-21-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-S6391091-A
titleOfInvention Production of menaquinone-4
abstract PURPOSE: To stably, readily, and industrially produce menaquinone-4, by culturing a bacterium having an ability to produce menaquinone-4 while controlling a dissolved oxygen concentration. n CONSTITUTION: A menaquinone-4-producing bacterium (e.g. Staphyloccus sciuri) is subjected to ultraviolet ray treatment, N-methyl-N'-nitro-N-nitrosoguanidine treatment and ethyl methanosulfonate treatment to provide the bacterium having ability to produce menaquinone-4. The bacterium is then inoculated in a medium to which alcohols (e.g. isopentenylalcohol), shikimic acid, D-succinyl benzoic acid, etc., are added and cultured at 5W8.5pH and 20W40°C with aeration without limiting the growth of the bacterium, while controlling a dissolved oxygen concentration in a culture medium so that the concentration increases after keeping 0W0.5ppm concentration in a logarithmic growth phase. n COPYRIGHT: (C)1988,JPO&Japio
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priorityDate 1986-10-07-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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