http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-S6384492-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2dcebf5a7f7ddc3b081f70e8ce1c4097 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-52 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-38 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate | 1986-09-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c6f2ee7c044b2bb23f910989a9ac94e2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1fe5984940c500e8c29fe56dd2541c6e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_db8a86b2fcb9569137a728a0f2545bb7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_83307c0b55b033825833ef9414780d5e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0f6fab10ec3d0731fee1ff64e02aa5eb |
| publicationDate | 1988-04-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-S6384492-A |
| titleOfInvention | Plasmid having ability to decompose vanillic acid |
| abstract | PURPOSE:To raise the productivity of an enzyme decomposing vanillic acid, by cleaving chromosome of Pseudomonas paucimobilis with a restriction enzyme and linking the formed fragment containing an enzyme gene capable of decomposing vanillic acid therein to a plasmid. CONSTITUTION:Cultivated microbial cells of Pseudomonas paucimobilis are treated to prepare a chromosomic DNA, which is cleaved with a restriction enzyme EcoRI to collect a fragment having enzymic gene capable of decomposing vanillic acid. The resultant fragment and a fragment obtained by cleaving a separately prepared plasmid pKT230 with the EcoRI are mixed and linked with a T4 ligase to afford the aimed plasmid having the ability to decompose vanillic acid. The resultant plasmid having the ability to decompose vanillic acid can be inserted into a host of Escherichia coli, bacterium of the genus Pseudomonas, etc., to provide a transformant. |
| priorityDate | 1986-09-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 26.