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filingDate 1986-08-26-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7f498b7472bd56fd2d20f2262b5f9d19
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publicationDate 1988-03-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-S6356294-A
titleOfInvention Production of carnitine
abstract PURPOSE:To obtain industrially carnitine having high optical purity at a low cost and in high yield, by reacting carnitine amide with a specific enzyme. CONSTITUTION:A microorganism which produces an enzyme capable of converting carnitine amide into carnitine is separated from the natural world to provided a strain of the genus Pseudomonas, CA32-C (FERM P-8911), which is subjected to shake culture at about 26 deg.C or 72hr in a culture medium containing glucose, meat extract, etc., and DL-carnitine amide hydrochloride, etc., to provided a culture medium containing an enzyme (amidase) having an activity by which carnitine amide is hydrolyzed to afford carnitine. Then, 0.1-30wt% carnitine amide (e.g. DL-carnitine amide hydrochloride) which is a substrate is added to the medium and reacted with the enzyme medium at 4-1 pH and 0.5-60 deg.C for 1-100hr. The resultant resultant liquid is treated with an ion exchange membrane, etc., to recover an optically active carnitine.
isCitedBy http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H08232371-A
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