| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5128cfc84a0fb189fceb3cd240c44493 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1241 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-85
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-865 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-32 |
| filingDate |
1987-06-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3cae82a69a20652069bdf0e7355b4b33
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_023f1199b3df20ac8f330e590b0774cc |
| publicationDate |
1988-12-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
JP-S63313594-A |
| titleOfInvention |
Production of cytidine diphosphocholine |
| abstract |
PURPOSE:To product CPD-choline in high efficiency, by using CMP and choline or phosphorylcholine as a substrate and contacting with a CPCT clone having increased activity of choline phosphate cytidyl transferase. CONSTITUTION:Whole DNA of Saccharomyces cerevisiae X2180-1 B is digested with a restriction enzyme SauBAI to obtain a DNA fragment of 5-15kb. A fragment produced by digesting vector YEp13 with BamHI is linked with the above DNA fragment using T4 ligase to obtain a recombinant DNA. A gene library of Saccharomyces cerevisiae is prepared by transforming E.coli K12 strain with the recombinant DNA. Saccharomyces cerevisiae D319-8A strain is transformed with said library to obtain CPCT clone. The cultured liquid, microbial cell or their treated product of the clone is made to contact with CMP (cytidine-5'-monophosphate) and choline or CMP and phosphorylcholine in a reaction liquid to obtain CDP-choline (cytidine diphosphocholine). |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7635691-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-1060215-C
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6930096-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0553821-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6387667-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CZ-300616-B6
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-0168064-A3 |
| priorityDate |
1987-06-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |