| abstract |
PURPOSE:To obtain a polypeptide having improved specific activity, improved stability, widened reaction spectrum and reduced side actions, by modifying a human tumor necrosis factor (TNF) protein into a specific amino acid sequence. CONSTITUTION:The objective physiologically active polypeptide has the amino acid sequence from the 1st Val to the 157th Leu shown in the figure, provided that one or more amino acid residues are substituted, depleted or inserted in the above sequence and at least the 32nd Arg is substituted to Glu. A polypeptide having Met bonded to the amino terminal is preferable. |