http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-S63245697-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ad2cd796f8c4dcb425ddcc68707d3444 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-32 |
| filingDate | 1987-03-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3a6d2fb28524bcfddc532e5ce7c0cb56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e96f52330ba184daf8ff4e48c03d485e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8aae49ff7d0e1550e98c94d6c86125d4 |
| publicationDate | 1988-10-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-S63245697-A |
| titleOfInvention | Method for measuring dehydrogenase or substrate |
| abstract | PURPOSE: To accurately, rapidly, continuously and automatically measure a dehydrogenase or substrate without occurrence of unnecessary depositing substances, etc., by (non)enzymically determining H 2 O 2 formed by specific enzymic reaction. n CONSTITUTION: A dehydrogenase, such as D-fructose dehydrogenase, without requiring a coenzyme, such as nicotinamide adenine dinucleotide (NAD + ), a substrate, such as D-fructose, and an electron carrier, such as 1-methoxy-5- methylphenazinum methyl sulfate (1-mPMS), in an amount of 0.0001W1mg/l are dissolved in a desired buffer solution to provide a reaction solution. The resultant reaction solution is reacted at about pH 4.5 and about 30°C for about 15min to accept electrons generated from the substrate through the electron carrier in O 2 and generate H 2 O 2 , which is then enzymically colored. The absorbance is subsequently measured to determine the dehydrogenase or sub strate. n COPYRIGHT: (C)1988,JPO&Japio |
| priorityDate | 1987-03-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 25.