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filingDate 1987-03-02-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 1988-09-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-S63215700-A
titleOfInvention Low-molecular weight protein
abstract NEW MATERIAL:A compound having the sequence of 1W24 amino acids from the side of the amino terminal expressed by the formula and the following physico-chemical properties. Molecular weight; 19,000±2,000 (measured by a gel filtration method using TSK gel G3000SW) and 16,000±2,000 [sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE)]. Isoelectric point; pH4.9±0.3. Ultraviolet absorption; Maximum value at 277nm and minimum value at 250nm. Aqueous solution; Weak acidic. Color reaction; Capable of assuming color reaction of peptide bonds and amino acids to the biuret reaction, Folin-Lowry reaction method and ninhydrin reaction after hydrolysis. n USE: An antitumor agent. n PREPARATION: For example, a human-derived cultivated and established lymphatic cell is added to a culture medium and an antitumor substance inducer is added to carry out cultivation. The resultant supernatant liquid is collected by centrifugation, subjected to ion exchange chromatography, ultrafiltered and concentrated and then subjected to gel filtration to afford the aimed low- molecular weight protein having antitumor activity. n COPYRIGHT: (C)1988,JPO&Japio
priorityDate 1987-03-02-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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