abstract |
PURPOSE:To obtain D-alanine useful as an intermediate for drugs or an intermediate for sweeteners efficiently, by cultivating specific yeast in a medium containing DL-alanine. CONSTITUTION:Yeast belonging to the genus Candida, Saccharomycospsis, Pichia, Torulopsis, Cryptococcus, Hansenula or Torycosporon, capable of assimilating L-alanine but substantially not D-alanine, is cultivated in a medium containing DL-alanine as a single carbon source and a single nitrogen source and D-alanine is collected from the culture mixture. Candida humicola ATCC36992, etc., may be cited as the yeast. |