| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e1ccbc69ae0050dd6561f4ce768a5994
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cd3ab617a6ad8610c63af5f5dfe97b0b |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-91 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-577
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-574
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-005
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-195
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00 |
| filingDate |
1986-10-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f0681a39139b050bf163788e1f1041b4
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7ff6ffe26a00f11377c855c0556fbe02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f8a7dc2a70ab12bf7aae6b38c39efaf0 |
| publicationDate |
1988-07-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
JP-S63173594-A |
| titleOfInvention |
Secretory protein induced by carcinogenic gene |
| abstract |
PURPOSE:To obtain a secretory protein induced by a carcinogenic gene and useful for the diagnosis and remedy of cancer, by specifying the molecular weight and isoelectric point of protein produced by culturing a cell originated from human hepatoma cell. CONSTITUTION:A human hepatoma cell (e.g. HuH-7cl-4) is inoculated in a medium in a Petri dish at a density of 0.5X10<4>-5X10<4> cells/cm<2> and cultured at about 37 deg.C for 1-4 days in an atmosphere having low oxygen partial pressure and a CO2 concentration of 3-10%. A plasmid (e.g. pSV2-myc) integrated with a carcinogenic gene [e.g. C-myc (a protooncogene corresponding to oncogene v-myc of chicken myelocytomatosis virus MC29)] is introduced into the obtained cultured cell. The introduced cell obtained by the above process is cloned and the separated cell is cultured in a medium such as Eagle MEM containing about 10wt.% bovine serum at a density of 10<4>-10<6> cells/cm<2> at about 37 deg.C for 2-4 days in an atmosphere containing 3-10wt.% CO2 to effect the secretion and production of protein in the medium. The objective protein having a molecular weight of about 46,000 and an isoelectric point of 7.1-7.3 can be separated from the cultured product after purification. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10166276-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6051237-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8114414-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10695410-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10189885-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8241636-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9499602-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8791237-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8771702-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9919038-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8956621-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7588930-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7700344-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8268326-B2 |
| priorityDate |
1986-08-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |