http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-S63152996-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c1755eede76ea7e719a3e0d2843cd793 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-32 |
| filingDate | 1986-12-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_07f5cfa664d34f0f02d6c361d1508c2a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_317eb3ddc98e6392b196c90e45d9c538 |
| publicationDate | 1988-06-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-S63152996-A |
| titleOfInvention | Fractionation and quantitative determination of bile acid |
| abstract | PURPOSE: To enable quantitative determination of various bile acids in a specimen in high accuracy in a short time, by using an internal standard substance obtained by the hydrolysis of a condensation product of free bile acid and an acidic amino acid ester. n CONSTITUTION: A specimen solution containing bile acids and an internal standard substance which is a hydrolysis product of a condensate of a free bile acid such as cholic acid and an acidic amino acid ester such as diethyl glutamate, etc., is supplied from a sampler 21 to a pretreatment apparatus 2. The bile acid-containing elute obtained by the retreatment is poured into a separation column 3 and the bile acids are separated into individual components and eluted. The elute is introduced into an immobilized enzyme column 5 together with an enzymatic reaction reagent solution 4 containing a substance capable of performing enzymatic reaction with bile acid, e.g. nicotinamide adenine dinucleotide. The immobilized enzyme column 5 contains immobilized bile acid dehydrogenase. The eluted liquid is introduced into a detector 6 and the quantity of NADH is determined to determine the objective bile acid. n COPYRIGHT: (C)1988,JPO&Japio |
| priorityDate | 1986-12-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 29.