| abstract |
PURPOSE: To industrially and advantageously obtain the titled compound useful as a synthetic raw material for various medicines etc., by affecting a dialkyl malate with an enzyme, microbial cell, etc., having the ability to selectively and asymmetrically hydrolyze ester bond at the α-position. n CONSTITUTION: A dialkyl malate, e.g. dimethyl malate, etc., is affected with an enzyme, microbial cell, e.g. Cryptococcus laurentii (ATCC 18803 strain), etc., having the ability to selectively and asymmetrically hydrolyze ester bond at the α-position or treated materials thereof to provide an optically active β-monoalkyl malate. Furthermore, the ester group of this compound is amidated to give optically active β-malamic acid, which is then reacted with an alkali hypohalite to afford optically active isoserine. n COPYRIGHT: (C)1988,JPO&Japio |