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filingDate 1986-11-07-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7fd475778651324700cdb9341b2010cf
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publicationDate 1988-05-07-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-S63102684-A
titleOfInvention Gene and use thereof
abstract PURPOSE:To obtain a DNA to code serrapeptase polypeptide useful for producing a large amount of serrapeptase by genetic engineering technology, prepared from chromosome DNA of a bacterium belonging to the genus Serratia capable of producing serrapeptase. CONSTITUTION:Chrmosome DNA is prepared from a cell of a bacteria (e.g. Serratia marcescens) capable of producing serrapeptase and scissored with a restriction enzyme. The prepared chromosome DNA fragment is linked to a vector (e.g. pBR322 or pUC12) by then use of DNA ligase. A host cell is transformed with the recombinant DNA. The prepared transformant is selected based on drug resistance of used vector as an index and then DNA having base sequence shown by formula I [X1 and X2 are T or G] and/or formula II (Y1 is T or G; Y2 is A, G, T or C; Y3 is T or G) is screened by the use of a prove to give a clone to hybridize the DNA.
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