http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-S62289200-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_527cbadc3d8cf81b5bf931c0dd99b3c1 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-48 |
| filingDate | 1986-06-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4096b863f201246eb75603ff46118de2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_68adc55f1f6c57cb2a79fbd8f7bb39b8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c72d2b74ab59547978d14ff6fb146ae3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bbb4c9573bbceff2af40f18703d3cdf6 |
| publicationDate | 1987-12-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-S62289200-A |
| titleOfInvention | Enzyme-labeling determination |
| abstract | PURPOSE: To enable the accurate and quick enzyme-labeling determination of trace substance useful in the fields of immunodiagnosis, biochemistry and fermentation industry, etc., by using a specific enzyme as a labeling substance. n CONSTITUTION: An enzyme (A) such as phosphoglucokinase is used as a labeling substance (enzyme) and bonded to an antigen, etc. The product is made to react with a substance (B) to be determined (antigen) and the reaction mixture is added with phosphoglucomutase (C), glucose 6-phosphate dehydrogenase (D), glucose 1-phosphate (E) and ATP and NAD(P)(F) to effect enzyme-A reaction. The component E is converted to glucose 6-phosphate with the enzyme C using glucose 1,6-2-phosphate (G) produced by the above reaction as a catalyst and, at the same time, the component F is converted to NAD(P)H(H) by conjugated reaction with the enzyme D. The enzymatic activity of the above substance B reaction product is determined from the absorptivity at 340nm originated from the resultant component H and the amount of the substance B is calculated from the activity. n COPYRIGHT: (C)1987,JPO&Japio |
| priorityDate | 1986-06-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 80.