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http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N31-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543
filingDate 1986-05-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1bf19523419fff6cecba58760b416081
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_75bc45f37e9bd0de7a4562c6aef5e6aa
publicationDate 1987-11-21-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-S62269065-A
titleOfInvention Multilayered analytical element and analysis of specimen occurring from living body using the same
abstract PURPOSE: To obtain a multilayered analytical element capable of performing measurement based on transmitted light, by inserting a liquid impervious film in the light pervious reagent layer laminated to a light pervious support in a detachable manner to partition the reagent layer into two upper and lower layers. n CONSTITUTION: A multilayered analytical element is formed by successively laminating a first reagent layer 2, a detachable liquid impervious film 3 and a second reagent layer 4 to a light pervious support 1. This element is used, for example, to perform the quantification of the rheumatoid factor in human serum. The reagent layer 2 comprises a mixture wherein 4-aminoantipyrine, phenol, glucose and glucose oxidase are contained in polyacrylamide and p-nonylphenoxyglycerine and the reagent layer 4 comprises a transparent porous film fabric having modified human IgG immobilized thereon. A liquid mixture of a definite amount of anti-human IG labelled with peroxidase and human serum is deposited on the layer 4 in a spot form and, after reaction, the film 3 is detached to move an unreacted substance to the layer 2. Absorbancy is measured by a spectrophotometer and the rheumatoid factor is quantified from a calibration curve. n COPYRIGHT: (C)1987,JPO&Japio
priorityDate 1986-05-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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