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filingDate 1986-05-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_10148012e194151529f8b88969cdad73
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publicationDate 1987-11-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-S62263457-A
titleOfInvention Preparation of immobilized urease membrane for urea sensor
abstract PURPOSE: To prepare a high sensitivity sensor, by using an aqueous polyvinyl pyrrolidone (PVP) solution having 2,5-bis-cyclopentanone disodium salt (BASC) added thereto as an urease immobilizing material to perform the irradiation of light. n CONSTITUTION: An aqueous solution containing urease is applied to a pH-ISFET chip 1. Thereafter, the gate surface part of a pH-ISFET element 2 is irradiated with light of an ultraviolet region using an exposure device. Successively, a part of the chip 1 of a sensor is immersed in a glutaraldehyde (GA) solution to be developed to obtain an immobilized urease membrane 3 only on the right- hand surface part. By this method, the membrane 3 rich in the immobilization quantity of urease is obtained and a high sensitivity sensor can be formed. n COPYRIGHT: (C)1987,JPO&Japio
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