http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-S62232378-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_80787665b837ed3eb503bbcd27c0043a |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00 |
| filingDate | 1986-04-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dfe2e1106f10aea9afdbf80a19bfc7de http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c6a8d5989c937421a8fe568aa8b55c63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f42ada6a37ad629350eeae2c030124b8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6bd25a104c92c80c422fa887f2327da4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_40a216db8c771186edc22585a0e63cb6 |
| publicationDate | 1987-10-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-S62232378-A |
| titleOfInvention | Fed-batch culture of gene recombinant cell |
| abstract | PURPOSE: To realize culture of gene recombinant bacterial cell in high cell concentration without causing falling-off of plasmid and to enable mass production of the objective substance, by successively adding a culture-suppressing substance and an agent for preventing falling-off of plasmid to the culture medium. n CONSTITUTION: A gene recombinant cell (e.g. E.coli) retaining a composite DNA composed of the objective gene, a vector and a promoter in the cell and capable of expressing said objective gene is subjected to fed-batch culture. In the above culture process, the culture system is continuously or intermittently added with (A) a substance suppressing said promoter (e.g. tryptophan when the promoter is tryptophan promoter) and (B) an agent corresponding to the drug-resistant gene code to said composite DNA (e.g. ampicillin, kanamycin, etc.). n COPYRIGHT: (C)1987,JPO&Japio |
| priorityDate | 1986-04-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 32.