| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_15fe1e4588b510351f85374c472475ca |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-4737 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-91 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-577
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-47
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-16
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-435
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-08
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-02 |
| filingDate |
1986-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_293d2cc0e6902a5d6e8b39fb3cc964b9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3235b7a147c728916e1ffb661fba5b4a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_674ea652b3bdc6a4f772f14945679fb7 |
| publicationDate |
1987-09-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
JP-S62210985-A |
| titleOfInvention |
Separation and purification of bovine c-reactive protein using hybridoma producing monoclonal antibody against bovine c-reactive protein and said antibody |
| abstract |
PURPOSE:To separate and purify a C-reactive protein which is an antigen in high purity and yield, by preparing a hybridoma producing an antibody specifically reactive with a bovine C-reactive protein and using the antibody. CONSTITUTION:A mouse is immunized with a mixture of a bovine C-reactive protein (hereinafter abbreviated to CRP) or a fraction containing the CRP with Freund's complete adjuvant and splenic lymphocytes collected from the spleen of the mouse are used to mouse myelomatous cells by a well-known method to give a hybridoma producing antibovine CRP antibody. A supernatant of a culture medium after cultivating the hybridoma is salted out with ammonium sulfate to afford a precipitated protein, which is then purified with a protein A-Sepharose CL4B or aninon exchanger to provide a monoclonal antibovine CRP antibody. The resultant antibody is then linked and immobilized with a carrier for affinity chromatography and CRP is then separated from bovine blood serum using a column of the above-mentioned carrier. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9012884-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/AU-631192-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5500345-A |
| priorityDate |
1986-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |