| abstract |
PURPOSE:To provide a gene containing one or more groups of restriction enzyme recognition sites selected from specific 4 kinds of groups and having a property characterized by the destruction of streptomycin-sensitive gene when a foreign DNA is integrated in the recognition site. CONSTITUTION:A streptomycin-sensitive gene containing one or more groups selected from (A) a restriction enzyme recognition site group composed of AsuII, SacI, BalI and XhoI, (B) a restriction enzyme recognition site group composed of MluI, SnaI, EcoRI, MstI and BstEII, (C) a restriction enzyme recognition site group composed of BclI, NotI and BglII and (D) a restriction enzyme recognition site group composed of KpnI, SacII, XbaI, SalI, ClaI, PvuI and StuI and preferably further containing a BamHI restriction enzyme recognition site. |