http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-S62149625-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_afd115dec46c157d3739020ebd4d866b |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-6456 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-49 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-46 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-72 |
| filingDate | 1985-12-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1c5a13ad829e03f2bdaf04b0646828a2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e7b9339aee8e29da2163363ebeb12f3b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8d749681548fabd8557c7185da8a7f7c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0763b0a0e067b974284a3f4dbbece75e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_721472018f22e46aa8f77ff94df2ed40 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_910b164eb3bf9c13dfb1ef29a087871a |
| publicationDate | 1987-07-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-S62149625-A |
| titleOfInvention | Production of physiologically active substance |
| abstract | PURPOSE: To obtain a physiologically active substance in a blood serum-free culture medium or culture medium of blood serum in a low concentration with a large amount of production without causing denaturation of products, by using a human kidney-derived established cell as a host for a gene recombination technique. n CONSTITUTION: A primary culture diploid cell obtained from a human fetus is subcultured in a blood serum-free culture medium containing an adequate amount (0.05W0.2wt/vol%) of added blood serum albumin or a culture medium containing added blood serum in a low concentration (0.05W0.2wt/vol%) to give the aimed human kidney-derived established cell. A cell capable of producing a desired physiologically active substance is preferably used as the above- mentioned cell. A vector for gene expression of the physiologically active substance is inserted into the above-mentioned established cell to carry out transformation and the resultant transformant cell is cultivated to recover the aimed physiologically active substance from the culture medium. Examples of the physiologically active substance expected to be produced by this method include tPA, urokinase, pro-UK, IFN-γ, or the like. n COPYRIGHT: (C)1987,JPO&Japio |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0303028-A1 |
| priorityDate | 1985-12-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 68.