http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-S62142272-A

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filingDate 1985-12-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a9635e92cac79d29226b5beb6610fb7d
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publicationDate 1987-06-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-S62142272-A
titleOfInvention Quantitative determination of ammonia or atp
abstract PURPOSE: To make quantitative determination with high sensitivity by acting glutamine synthesizing enzyme to a specimen liquid in the presence of another material which is not the component to be quantitatively determined and acting purine nucleoside phosphorylase thereto in the presence of purine nucleoside then making quantitative determination with xanthine oxidase. n CONSTITUTION: The glutamine synthesizing enzyme is acted to the specimen liquid in the presence of another component which is not the component to be determined, for example, ATP in the case of determining ammonia and ammonia in the case of determining ATP as well as the L-glutamic acid in the case of quantitatively determining the ammonia or ATP in the specimen liquid. The purine nucleoside phosphorylase is acted to the formed inorg. phosphoric acid and the formed purine compd. is quantitatively determined by the xanthine oxidase. Two molecules of hydrogen peroxide are formed from one molecule of the ammonia or ATP if the xanthine oxidase is used and therefore, the sensitivity is made two-fold higher and the quantitative determination with the higher sensitivity is made possible. n COPYRIGHT: (C)1987,JPO&Japio
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