| abstract |
PURPOSE: To produce the titled compound economically in an industrial scale, by bonding N-protected proline to an amino group-containing resin, bonding the protected amino acids of from the 31-site to 1-site successively by solid-phase synthesis, removing the resin and the protecting groups, and forming a disulfide bond between the 1-site and the 7-site. n CONSTITUTION: An N-protected proline is bonded to an insoluble resin having amino group (e.g. benzhydrylamine resin), and the protected amino acids of chicken calcitonin from the 31-site to the 1-site are bonded successively by solid- phase synthesis. The product is treated with hydrogen fluoride to remove the insoluble resin and the protecting groups of the amino groups to obtain the calcitonin precursor of formula I. The objective compound of formula II can be prepared by bonding the cysteines of the 1- and 7-sites via their mercapto groups to form a disulfide bond. n COPYRIGHT: (C)1986,JPO&Japio |