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filingDate 1985-05-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_45b25f7a0cb04088394b741a8b6fb840
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publicationDate 1986-11-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-S61268196-A
titleOfInvention Production of ursocholic acid
abstract PURPOSE: To produce ursocholic acid stably and economically with simple procedure, by contacting deoxycholic acid with one or more kinds of microbial strains such as Pleurotus genus. n CONSTITUTION: On or more kinds of microbial strains capable of converting deoxycholic acid to ursocholic acid and listed in the Table are cultured in a liquid medium consisting of a carbon source (e.g. xylose), a nitrogen source (e.g. peptone), vitamins, various inorganic salts, etc., at 2W10pH and 15W35°C for 2W14 days (for bacidiomycetes) or 2W10 days (for fungi). After cultivation, the microbial cells are collected, washed with water, suspended in water or a buffering solution and added with deoxycholic acid to effect the sufficient contact with said microbial cells. A hydroxyl group is attached to the 7β-site of deoxycholic acid and ursocholic acid is produced according to the reaction mechanism of the Figure. The produced ursocholic acid is extracted, separated and purified. n COPYRIGHT: (C)1986,JPO&Japio
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