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filingDate 1985-02-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_43b7075b8d4ae002e8de08bfd3bc5c9b
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publicationDate 1986-08-21-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-S61187656-A
titleOfInvention Detection of fluorescence by stable reagent
abstract PURPOSE: To attain to enhance reliability, by preparing a reagent by dissolving 2-mercaptoethanol used as a thiol compound in water or an aqueous boric acid solution apart from other reagent and using all of reagents by simultaneously mixing the same. n CONSTITUTION: In a method wherein o-phthanolaldehyde is used to be reacted with primary amine in the presence of a thiol compound to form an isoindole type fluorescent compound and the fluorescence of this compound is detected to perform the quantitative analysis of primary amine, 2-mercaptoethanol used as the thiol compound is dissolved in water or an aqueous boric acid solution to prepare a reagent separated from other reagent and said prepared reagent is preserved and all of the reagents are simultaneously mixed at a use time. By this method, the daily deterioration of the reagents are suppressed and the reliability of a measured can be enhanced. n COPYRIGHT: (C)1986,JPO&Japio
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