| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fc3e5cb7a2da33ea01dae32f31bef542 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S530-809
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S530-832 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-18
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-79 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-00 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-79
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-08
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-18
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-16
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-435
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-00 |
| filingDate |
1984-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8e3772597b0eaee88e45d7824cd863a6
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fd11e04df1c0c50673bd17923fe9e02a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_52becc2322aa8622808c034039ea0b58
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5722bff642c70a8e2d33a717b0fc20d5 |
| publicationDate |
1986-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
JP-S61145200-A |
| titleOfInvention |
Separation and purification of bovine lactoferrin |
| abstract |
PURPOSE: Cow milk is passed through an affinity column filled with insoluble support to which monoclonal antibovine lactoferrin antibody is immobilized to enable high-yield separation and high-purity purification of the titled substance which binds to iron, thus having bacteriostatic action on iron-requiring bacteria. n CONSTITUTION: Mouse spleen lymphocytes immunized with bovine lactoferrin are fused with mouse myeloma cells and the resultant hybridoma is cultured to produce monoclonal antibovine lactoferrin antibody. The resultant antibody is immobilized on a support for affinity chromatography by stirring them overnight. Cow milk is passed through the column to effect adsorption of bovine lactoferrin, which is then eluted with an eluent of a less than 4.7 pH, preferably 4.3W2.7 pH, then the eluted fraction is immediately neutralized to effect isolation and purification of the objective substance. n COPYRIGHT: (C)1986,JPO&Japio |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0385118-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-4988629-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H02225419-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H02138295-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2016117750-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9804922-A1 |
| priorityDate |
1984-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |