| abstract |
NEW MATERIAL:A compound bearing a labeled purine base analog of formula I (X 1 , X 2 , X 3 are C, N; Y, Z are H, hydroxyl, amino group; F is fluorescent labeling compound) where 7-deazapurine is excluded. n USE: A clinical diagnostic reagent used in identification of labeled genes, a reagent in biotechnology for extracting genes. n PREPARATION: For example, 2'-deoxyaribonucleoside transferase and thymidine are allowed to act on 4-amino-3(2-aminoethyl)-pyrazolo[3,4-d]pyrimidine and further phosphorus oxychloride is allowed to react with the product in anhydrous trimethyl phosphate to give 5'-monophosphate of formula III. The product is converted into 5'-triphosphate, which is used to effect nick translation whereby a polynucleotide bearing the group of formula I is obtained. n COPYRIGHT: (C)1986,JPO&Japio |