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filingDate 1984-05-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1e784fb3c8335e6d42b61844e6777294
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publicationDate 1985-11-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-S60239500-A
titleOfInvention Preparation of tyrosyl oligopeptide having radioactive iodine imparted thereto
abstract PURPOSE: To obtain a tyrosyl oligopeptide in high yield with high reproducibility and carry out easily the subsequent radioactive iodination process, by reacting tyrosine with an oligopeptide at a selected charging molar ratio. n CONSTITUTION: (C) An oligopeptide, e.g. synthetic human C-peptide, is reacted with (D) tyrosine in a molar amount within 0.01W10mol range based on one mol component (C) in the presence of (A) tyrosyl-tRNA synthetase (an enzyme catalyzing the reaction; tyrosine+ATP+tRNA→tyrosyl-tRNA+AMP+pyrophosphoric acid, optimally obtained from thermostable bacteria, e.g. Bacillus thermophilus) and (B) adenosine triphosphate, preferably at 1:1W1:100 molar ratio between the components (A) and (B) to give a tyrosyl oligopeptide, which is then iodinated with radioactive iodine ( 125 I) to afford the aimed substance. n COPYRIGHT: (C)1985,JPO&Japio
priorityDate 1984-05-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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Total number of triples: 33.