| abstract |
NEW MATERIAL:A thrombin binding substance derived from humans. Molecular weight; 88000±10000 in the reduced state, and 71000±10000 in the nonreduced state. Isoelectric point; 4.2±0.5pH. Affinity; Strong affinity for thrombin. Stability; Stable at 2W10pH and stable to a modifier, e.g. sodium dodecyl sulfate or urea, and treatment with pepsin. n USE: A fibrinolytic promoter and anticoagulant capable of bonding with thrombin to enhance specifically the activation of protein C and prolonging the blood coagulation time. n PREPARATION: A pulverized human placenta is extracted with a buffer solution, e.g. tris hydrochloric acid buffer solution, containing a nonionic surfactant, e.g. Triton X-100 or Lubrol PX, and the resultant extract is then separated and purified by affinity chromatography with a conjugate of thrombin with a carrier, e.g. diisopropylphosphoro-thrombin-agarose, to give the titled substance. n COPYRIGHT: (C)1985,JPO&Japio |