| abstract |
PURPOSE:To obtain a biosensor which can measure easily and quickly the specific component in the sample of a living body with high accuracy by providing a measuring electrode and counter electrode on an insulating substrate and coating the electrode system with a prescribed reactive layer and porous filter layer. CONSTITUTION:Platinum is embedded into an insulating substrate 10 consisting of PVC to provide a measuring electrode 11 and a counter electrode 12. A nonwoven fabric 13 is installed to cover the electrode system. The fabric 13 carries glucose oxidase 14 as an oxidation reduction enzyme and potassium ferricyanide as an oxidation type dye to be conjugated to the oxidation reduction enzyme in a dry state after said fabric is impregnated into the soln. thereof. A filter layer 16 consisting of porous polycarbonate is installed on the fabric 13. A blood is added to such sensor and the potential of the electrode 11 is swept (0-0.5V) on the basis of the electrode 12, then the oxidation current is measured, by which the concn. of the glucose in the blood is measured. The biosensor which can measure easily and quickly the specific component in the sample of a living body with good accuracy is thus obtd. |