http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-S60137300-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2893e0867aa8119b9e5ebdb5949773a6 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-74 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-34 |
filingDate | 1983-12-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eb289b5c392b6f28ebbfe58868509631 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_de0b88121954ff68153e481b24cc46ce http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ad0cee1b1d215f41d77daebd2e0f5fea |
publicationDate | 1985-07-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-S60137300-A |
titleOfInvention | Determination of 3alpha-hydroxysteroid |
abstract | PURPOSE: 3α-Hydroxysteroid (bile acid) in the sample to be tested is converted into nicotin-adenine nucleotide of reduced type (NADH), then a metal ion is allowed to act on the NADH and the amount of the metal complex formed is determined, thus even a trace amount of sample has become determinable. n CONSTITUTION: 3α-Hydroxysteroid dehydrogenase (3α-HSD) is allowed to act on 3α-hydroxysteroid in the sample to be determined in the presence of nicotin- adenine nucleotide (NAD) to form NADH. Then, the electrons of NADH are allowed to act on metal ion using an electron carrier such as 1-methoxy-5-methylphenazinium methyl sulfate in the presence of an organic acid salt oxamate or oxalate to form a reduced metal ion and a specific metal color reaction reagent is allowed to act on the ion to form the metal complex and the complex is determined. n COPYRIGHT: (C)1985,JPO&Japio |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-S62195559-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-100454002-C |
priorityDate | 1983-12-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 36.