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filingDate 1982-08-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 1984-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-S5935142-A
titleOfInvention Determination of catecholamine
abstract PURPOSE:To achieve a highly accurate determination, by a method wherein dansylated catecholamine prepared by the dansylation of catecholamine is subjected to liquid chromatography to separate various derivatives, upon which an oxalic ester and H2O2 are made to act to detect light emission. CONSTITUTION:A dansyl halide is added to a sample containing catecholamine in a biological fluid such as blood or urine to form a catecholamine derivative. The resulting sample solution is fed with a metering pump 2 to a sample injector 3 and mixed with an eluent fed to the sample injector 3 from a storage tank 1. The mixture is sent to a column of high performance chromatography to separate three components of noradrenaline, adrenaline and dopamin each of which has been dansylated. Solutions are added to the separated samples from an oxalic ester solution tank 5, an H2O2 solution tank 7 and a buffer tank 9, respectively, and fluorescence generated is detected with a detector 11 to be recorded on a recorder 12. This enables the simultaneous determination of the three trace components with a high accuracy and for a short time.
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