| abstract |
PURPOSE: To determine directly creatinine existing in a specimen containing ammonia, by using a combination of specific enzymatic reaction systems. n CONSTITUTION: (i) Glutamic dehydrogenase (GlDH), α-oxoglutaric acid (α-OG), reductive nicotinamide adenine dinucleotide phosphate (NADPH), and an enzyme and substrate [e.g., glucose-6-phosphate dehydrogenase (G-6-PDH) and glucose- 6-phosphate(G-6-P)] to reduce nicotinamide adenine dinucleotide phosphate (NADP + ) are added to a specimen, all NH 3 existing in a mixed solution is consumed, and, simultaneously, formed NADP + is converted into NADPH. (ii) Reductive nicotinamide adenine dinucleotide phosphate (NADH) and creatinindeiminase are added to the mixed solution to carry out the reaction, and the reduction in amount of NADH is measured to determine creatinine amount. n COPYRIGHT: (C)1984,JPO&Japio |