http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-S59162899-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_be3928f66bb2545a8175c61c56146b9a http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d913bbc990f78742487267dda0639e89 |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-805 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-32 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-26 |
filingDate | 1983-03-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_60614d61102f53da7cfc89097f36aec2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4b68f13295d5042bd71b8502797bcb55 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d24d6a9249e4167ef774ffee69b6639d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c818498d4f05736609b77537499bdee6 |
publicationDate | 1984-09-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-S59162899-A |
titleOfInvention | Method and composition for determination of beta-hydroxybutyric acid |
abstract | PURPOSE: To determine β-hydroxybutyric acid easily and rapidly, by reacting β-hydroxybutyric acid with NAD in the presence of a dehydrogenase, reducing a tetrazolium salt with the produced NADH, and determining the amount of the produced formazan by colorimetry. n CONSTITUTION: β-Hydroxybutyric acid in a specimen is oxidized to acetoacetic acid with β-hydroxybutyric acid dehydrogenase in the presence of nicotinamide adenine dinucleotide (NAD). The tetrazolium salt added to the system {e.g. 3,3'- (3, 3'-dimethoxy-4, 4'-biphenylene)-bis[2-( p-nitrophenyl )-5-phenyl-2H-tetrazolium]} is reduced by the reducing NAD (NADH) produced by the above oxidation reaction, by the aid of an electron transfer material (e.g. methyl-1-methoxy-5-methylphenazium sulfate), and the produced colored formazan is determined by colorimetry to determine the β-hydroxybutyric acid (cf. reaction formula). n COPYRIGHT: (C)1984,JPO&Japio |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H01117799-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2015049050-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-S63212868-A |
priorityDate | 1983-03-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 40.