http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-S59118718-A
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_983fa366d54b080542742d72951c002c |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-395 |
filingDate | 1982-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7ea1d277f1da1ea0277a83e032b61997 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9c9f97077fb72e0adc261ad12da434df |
publicationDate | 1984-07-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-S59118718-A |
titleOfInvention | Recovery of igm |
abstract | PURPOSE: To prepare IgM easily, in high yield, by separating albumin and IgG fraction from blood plasma, extracting IgM from the above residue, adding polyethylene glycol to the extracted liquid, and recovering the produced precipitate. n CONSTITUTION: Albumin and IgG fraction are separated from blood plasma by Cohn's ethanol fractionation and acrinol fractionation. The obtained residue is dispersed in a buffer solution such as acetic acid buffer solution having 4W 7 pH, or water, physiological saline water, etc., stirred, and separated into solid and liquid to extract IgM. The extracted liquid is adjusted to the protein concentration of 1W5% and pH of 4.3W6, preferably 4.5W5, mixed with polyethylene glycol, stirred at 10W20°C, and left to stand for about a day, and the produced precipitate is recovered. The obtained precipitate is treated with dextran sulfate and calcium chloride to obtain IgM free from foreign materials. n COPYRIGHT: (C)1984,JPO&Japio |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H0278635-A |
priorityDate | 1982-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 37.