http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-S59109861-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f97f0fa258a008bf056ad04b7f380dda |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-74 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-74 |
| filingDate | 1982-12-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_181651ba950ad11fda302142f2fd08b7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3ba33f0298974eb3a92941d4b1425717 |
| publicationDate | 1984-06-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-S59109861-A |
| titleOfInvention | Method and device for analyzing homovanilic acid and vanilylmandelic acid |
| abstract | PURPOSE:To analyze homovanilic acid and vanilylmandelic acid with high accuracy by separating both acids from a soln. to be analyzed using a moving phase contg. N(C4H9)4, producing each dimer in the presence of of H2O2 with peroxidase, and measuring fluorescent light intensity of each dimer. CONSTITUTION:A moving phase contg. N(C4H9)4 is fed from a vessel 7 together with a sample from a sample introducing part to a reverse phase chromatographic column 4 to separate a vanilylmandelic acid (VMA) and homovanilic acid (HVA). The eluate is fed to a column 6 is mixed with a phosphoric acid buffer soln. contg. peroxidase fed from a vessel 7A and a phosphoric acid buffer soln. contg. H2O2 fed from a vessel 7B and reacted to form each dimer of the 2 acids. They are fed to a mixing coil 9 together with a mixed soln. of boric acid-sodium carbonate-NaOH of pH11-12 fed from a vessel 7C. Since the produced dimers exhibit fluorescent light at lambdaEx 315nm and lambdaEm 425nm, respectively, said lights are detected with a detector 10 and indicated with a recorder 11. Said acids are separated and determined thus exactly in a short time. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H01131157-U |
| priorityDate | 1982-12-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 28.