| abstract |
PURPOSE: To prepare efficiently an amino acid, particularly L-cysteine or L- cystine, by adding 2-aminothiazoline-4-carboxylic acid to a microorganism in the logarithmic growth phase thereof. n CONSTITUTION: A microorganism, belonging to the genus Achromobacter, Alcaligenes, etc., and having the ability to hydrolyze 2-aminothiazoline-4-carboxylic acid (ATC) to produce L-cysteine or L-cystine is cultivated in a nutrient culture medium, and the ATC is further added to the culture medium in the logarithmic growth phase, particularly in the latter half of the logarithmic growth phase. The cultivation is further continued, and the culture fluid is kept at 30W45°C for at least 1hr or longer, preferably 1W3hr and matured to incrsase the enzymic activity and give the aimed stabilized culture fluid. The ATC is further added to the resultant culture fluid and reacted at about 15W60°C, preferably about 30W50°C, and 6W10pH, preferably about 7.0W9.5pH to collect the aimed L- cysteine or L-cystine from the reaction solution. n COPYRIGHT: (C)1983,JPO&Japio |