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filingDate 1981-07-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 1982-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-S5758893-A
titleOfInvention Analytical composition containing choline oxidase
abstract PURPOSE: An analytical composition for choline, betaine or a derivative thereof containing a novel choline oxidase, 4-aminoantipyrine, peroxidase, etc. as a color reagent. n CONSTITUTION: A novel choline oxidase is collected from cultivated Arthrobacter globiformis B-0577 (FERM-P No.3518) by the conventional cultivating and collecting method. The resultant choline oxidase is then added to a solution of choline or a derivative thereof, e.g. lysolecithin, sphingomyelin or bytyrylcholine, or betaine aldehyde or a derivative thereof in an aqueous medium. The resultant hydrogen peroxide formed by the enzymic reaction is then determined by a peroxidase, etc. or the formed betaine is similarly determined by the Reinecke salt method. Alternatively, the reduced oxygen may be determined by an oxygen electrode. n COPYRIGHT: (C)1982,JPO&Japio
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type http://data.epo.org/linked-data/def/patent/Publication

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