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filingDate 1980-09-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 1982-04-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-S5756430-A
titleOfInvention Live virus vaccine for canine parainfluenza
abstract PURPOSE: To provide a live virus vaccine effective to the respiratory diseases caused by infection with canine parainfluenza viruses and having high safety, by usig attenuated virus obtained by the extended tissue subculture of viruses. n CONSTITUTION: Viruses separated from a dog affected with respiratory diseases through the first-generation dog kidney (DK)culture cells, is subcultured for four generations in DK culture cells, and then subcultured in the established cells of the kidney of green monkey (Cerocopithecus aethiops sabaeus)(Vero cell) at 30±5°C. for ≥30 generations. The resultant viruses are inoculated in Vero cells, and cultured at 30°C. At the peak of the proliferation of the virus, the infected culture liquid is collected, mixed with the sterilized liquid consisting of a protecting agent (e.g. lactose) and purified water, and freeze-dried to obtain the titled live vaccine. It has remarkable effectiveness after three weeks from inoculation of the vaccine even by the aggresive test using the attenuated CPI virus. The vaccine gives no side effect in the test using a dog negative to antibody, and the high safety. n COPYRIGHT: (C)1982,JPO&Japio
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http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7682619-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-S60206584-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2009506050-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H033548-B2
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