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filingDate 1981-02-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_22ba5193d874ef774949571672fee58e
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publicationDate 1982-08-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-S57139095-A
titleOfInvention Plasmid vector
abstract PURPOSE:A plasmid vector, prepared by inactivating the immunological function for colicin E1 on ColE1 plasmid, and varying a gene in the colicin E1 protein to produce the colicin E1 vigorously at a specified temperature, and capable of remarkably increasing the amount of the plasmid multiplied in the host microorganism. CONSTITUTION:An exogenous gene capable of taking part in the tolerance to ampicillin transposon and kanamycin is inserted in a gene capable of taking part in the production of a protein capable of exhibiting the immunity against the colicin E1 on ColE1 plasmid to inactivate the immunological function. The resultant plasmid or a host microorganism containing the plasmid is then irradiated with ultraviolet light or brought into contact with a variation inducing agent, e.g. N-methyl-N'-nitro-N-nitrosoguanidine, to give a plasmid vector varied to produce colicin E1 vigorously at 37 deg.C even when the colicin E1 production is not induced.
priorityDate 1981-02-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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Total number of triples: 28.