http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-S5710453-A
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4b18d70d8bf538fb533d0aa5cf87170d |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-34 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-536 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-34 |
filingDate | 1981-04-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f76327bf133ecf490ddf45b5a7524392 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_14e1d925f04a7ab40be3c6253586a776 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_70e6a856c5bee37d172eb095e2bec82d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2fda9de294578d8fb948b277b26d444e |
publicationDate | 1982-01-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-S5710453-A |
titleOfInvention | Method for improving immunity determination |
abstract | PURPOSE:To determine the activity of bound beta-galactosidase immune component in enzyme immuno assay by using a reaction medium containing at least one compound selected from among hydrophilic alcohols and ketones. CONSTITUTION:0.2ml Of 0.1M phosphoric acid buffer solution of pH 6.8 (containing 1mM MgCl2; 0.1M NaCl; 0.1% cattle serum albumin; 0.1% NaN3), 10mg/ ml nitrophenyl beta-D galactoside (buffer solution) and a reaction medium or a reference medium are mixed. The mixture is subjected to reaction for 50min in a water bath 37-45 deg.C. At the end of the reaction the reaction is interrupted by adding 2.5ml of 0.1M glycine-sodium carbonate buffer solution of pH 10.4 to the mixture and the light absorbance of the mixture is measured at 420nm to determine the enzyme activity (relative activity). |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9303365-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0075293-A2 |
priorityDate | 1981-04-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 165.