| abstract |
PURPOSE: To determine activity of angiotensinase with ease, by blending a specific reagent with a solution containing angiotensinase to give quinoneimine dye, followed by measuring its concentration by colorimetry. n CONSTITUTION: A reagent consisting of a X-hippuryl-L-histidyl-L-leucine shown by the formula [X is H, OH, NH 2 , or N(CH 3 ) 2 ], hippuricase, peroxidase, 4-amino-antipyrine, and H 2 O 2 is added to a solution containing angiotensinase to form quinoneimine dye. The concentration of the dye is measured by colorimetry to determine activity of angiotensinase. n COPYRIGHT: (C)1981,JPO&Japio |