http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-S56127100-A

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filingDate 1980-03-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 1981-10-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-S56127100-A
titleOfInvention Determination of component in living body fluid
abstract PURPOSE: To eliminate the interfering substance in a specimen, by separating the substrate or enzyme which reacts with the objective component and the coupler of oxidative condensation reaction from 4-aminoantipyrine and peroxidase, and carrying out the determination by redox reaction. n CONSTITUTION: In the colorimetric determination of the objective component by the oxidative condensation 4-aminoantipyrine, a specific coupler, and hydrogen peroxide produce by redox reaction, in the presence of peroxidase, the reagent is divided into a component containing the coupler and a substrate or enzyme which reacts directly with the objective component and the second component containing 4-aminoantipyrine and peroxidase. The interfering substance in the specimen is removed by the latter reagent before determination. The interfering substance is, e.g. glycerol in the triglyceride determination, maltose and glucose in the α-amylase activity determination, etc. n COPYRIGHT: (C)1981,JPO&Japio
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