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filingDate 1980-02-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_583e7a2ff933437e21f542bc88b91dbf
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publicationDate 1981-09-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-S56117795-A
titleOfInvention Preparation of l-glutamic acid by fermentation method
abstract PURPOSE:To obtain L-glutamic acid in high yield, by using a transformation strain obtained by introducing a plasmid including a deoxyribonucleic acid carrying genetic information participating in the formation of L-glutamic acid prepared from a bacterium belonging to the genus Escherichia into a variant of the same genus. CONSTITUTION:A chromosome DNA is extracted conventionally from a strain having high L-glutamic acid producing ability, a bacterium belonging to the genus Escherichia, it is treated with an endonuclease, and it is blended with a DNA of plasmid or phage which is to be used as a vector treated similarly and united by a ligase. The pepared united product is included in a mold of the genus Escherichia by transformation and propagated until it is stabilized as a heredity character, to give a transformation strain. A strain resistant to p-fluorophenylalanine, S-(2- aminoethyl)-cystein, 2-thiazolealanine, and 1,2,4-triazolealanine is used as the competent bacterium.
priorityDate 1980-02-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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