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filingDate 1978-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 1980-01-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-S5576-A
titleOfInvention Novel lactic acid oxidase, its preparation, method of analysis using the same, and kit for above analysis
abstract NEW MATERIAL:A lactic acid oxidase characterized by: substrate specificity, L-lactic acid; enzymatic activity, L-lactic acid + O 2 → pyruvic acid + H 2 O 2 ; molecular weight 80,000±10,000; isoelectric point, about pH 4.8±0.3. n USE: Determination of purity of lactic acid reagent; quantitative analysis of lactic acid in blood, lactic acid derivatives, etc. Optimum conditions: pH6W7 and about 35°C. n PROCESS: Lactic acid oxidase-producing bacteria belonging to Pediococcus, Streptococcus, etc., are cultured, and the cells are recovered by centrifuging the cultured product. A solution containing lactic acid oxidase can be obtained by mechanical treatment, etc. of the cells. The solution is subjected to concentration, salting-out, freeze-drying, etc. to obtain the purified lactic acid oxidase. n COPYRIGHT: (C)1980,JPO&Japio
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Total number of triples: 24.