http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-S5534008-A
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_527cbadc3d8cf81b5bf931c0dd99b3c1 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-40 |
filingDate | 1978-08-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bbb4c9573bbceff2af40f18703d3cdf6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_708430abd9173ed5a234623b0f79ea1f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0b3a6caed703add9ff47b8f5ae24390e |
publicationDate | 1980-03-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-S5534008-A |
titleOfInvention | Determination of amylase activity |
abstract | PURPOSE: To determine the amylase activity with ease and accuracy, by converting maltose and glucose in the specimen with an enzyme system into 6-phosphogluconic acid, and by reacting the acid with dextrin, etc. and NAD + to increase the NADH. n CONSTITUTION: An enzyme system comprising maltase, hexokinase, glucose-6- phosphate dehydrogenase, an enzyme for oxidizing NAD + and NADPH, and a substrate for oxidizing NADPH is added to remove the maltose and (or) glucose from the specimen. The maltose and glucose are converted into 6-phosphogluconic acid. One or more types of starch, dextrin, amylose, amylopectin in excess, and NAD + are reacted with the acid to determine the amylase activity from the increase in NADH with an abosrbance at 340nm. n COPYRIGHT: (C)1980,JPO&Japio |
priorityDate | 1978-08-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 51.