http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-S5534008-A

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classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-40
filingDate 1978-08-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bbb4c9573bbceff2af40f18703d3cdf6
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publicationDate 1980-03-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-S5534008-A
titleOfInvention Determination of amylase activity
abstract PURPOSE: To determine the amylase activity with ease and accuracy, by converting maltose and glucose in the specimen with an enzyme system into 6-phosphogluconic acid, and by reacting the acid with dextrin, etc. and NAD + to increase the NADH. n CONSTITUTION: An enzyme system comprising maltase, hexokinase, glucose-6- phosphate dehydrogenase, an enzyme for oxidizing NAD + and NADPH, and a substrate for oxidizing NADPH is added to remove the maltose and (or) glucose from the specimen. The maltose and glucose are converted into 6-phosphogluconic acid. One or more types of starch, dextrin, amylose, amylopectin in excess, and NAD + are reacted with the acid to determine the amylase activity from the increase in NADH with an abosrbance at 340nm. n COPYRIGHT: (C)1980,JPO&Japio
priorityDate 1978-08-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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