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classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-64
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-77
filingDate 1978-07-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9813d905df53d9b10624b4b4da88766f
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publicationDate 1980-02-01-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-S5515004-A
titleOfInvention High-sensitive and simple coloring calculation method of aceto-acetic acid in blood
abstract PURPOSE: To calculate aceto-acetic acid in blood with a high sensitivity simply by making aceto-acetic acid in blood into an azo compound by reaction on p-nitropheny diazonium fluoborate and converting this azo compound to a hydrazo compound and comparing color. n CONSTITUTION: Albuminous substances are eliminated from the blood serum of a tested person, and aceto-acetic acid is made into an azo compound by reaction on p-nitrophenyl diazonium fluoborate in citric acid buffer liquid of pH 4.5. Next, phosphorous acid is added to decompose an excess diazonium salt and convert the azo compound to a hydrazo compound, and this solution has the photo absorptivity measured by a wave length of 390nm, and the measured value is compared with a standard calculation line, thereby calculating aceto-acetic acid in blood. In the figure, the standard calculation line aceto-acetic acid is shown and indicates a clear linearity. n COPYRIGHT: (C)1980,JPO&Japio
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