| abstract |
PURPOSE: To prepare the title compound useful as an intermediate for the synthesis of antibiotics, in high purity, by adsorbing a crude 7-aminocephem compound containing 7-amino-cephalosporanic acid (7-ACA) with specific resin, and eluting only the impurity with aqueous solution of NaCl. n CONSTITUTION: 7-Amino-3-(1-methyl-1H-tetrazol-5-ylthiomethyl)-3-cephem-4-carboxylic acid (7-ACA-TE) is prepared by reacting 7-ACA with 1-methyl-5- mercapto-1H-tetrazole. The 7-ACA-TE thus obtained is adsorbed from an aqueous solution of pH6.5W8.5 with a nonionic resin comprising a cross-linked copolymer of styrene-divinyl benzene, an eluted with 0.2W3.0 M NaCl solution. The impurities such as 7-ACA, etc., are selectively eluted. The remaining 7-ACA-TE is eluted with a solvent such as methanol, etc. and crystallized, etc., to obtain the high purity 7-ACA-TE. n COPYRIGHT: (C)1979,JPO&Japio |