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filingDate 1978-03-29-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_01875814d55499ae823bd9b5f1c82f10
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publicationDate 1979-10-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-S54129188-A
titleOfInvention Novel hydroxyglutaric acid dehydrogenase and its preparation
abstract NEW MATERIAL:Hydroxyglutaric acid dehydrogenase, an enzyme having catalytic acitivity to the reaction of formula I; substrate specificity to react specifically with D-α-hydroxyglutaric acid (D-HG) and α-ketoglutaric acid (α-KG); electron acceptor, nicotinamide adenine dinucleotide only; optimum pH ranges, 9.3W9.6 for α-KG → D-HG reaction, and 8.5W9.0 for D-HG → α-KG reaction; Molecular weight, 53,000±2,000 by ultracentrifugal sedimentation equilibrium analysis; subunit molecular weight, 32,000±1,000 by SDS electrophoresis; protein characteristics, dimer of the subunit; colorless, clear thin plate crystal by ammonium sulfate precipitation; sendimentation constant, S = 4.2±0.15 by ultracentrifugal analysis. n USE: Determination of α-KG, D-HG, etc. n PROCESS: Culturing of bacteria capable of producing said enzyme and belonging to Peptococcus genus, e.g. Peptococcus aerogenes ATCC 14963. n COPYRIGHT: (C)1979,JPO&Japio
priorityDate 1978-03-29-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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