| abstract |
(57) [Summary]nThe present invention provides a method of producing a viral gene delivery vehicle that can be introduced into a preselected cell type by using a targeting conjugate. The gene delivery vehicle comprises: 1) a gene of interest (to be delivered); 2) a viral capsid or an envelope that retains a member of a specific binding pair (the partner portion of which directly binds to the surface of the target cell). No). These vehicles can be rendered incapable of binding to their natural receptors on the cells. Targeting conjugates consist of a partner member of a specific binding pair that binds to a targeting moiety that is a ligand (or fragment thereof) specific for the cell type. Specific binding pair members present on viral vehicles include, for example, immunoglobulin binding moieties (eg, protein A, protein G, FcR, or anti-Ig antibodies capable of binding Fc fragments), or biotin, avidin or streptavidin. It can be. The outer membrane or capsid of the virus can contain substances that mediate entry of the gene delivery vehicle into target cells. Due to the specificity of the ligand, the high affinity of the binding pair, and because the gene delivery vehicle is not targeted when used alone, the universality of the method of gene delivery is due to the high selection of the cell type It can easily be achieved by the use of various suitable targeting conjugates, as well as by nature. |